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ID4 ELISA Kit (Human) (OKCA00660)

96 Wells
$600.00
In Stock
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Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
ID4
Official Gene Full Name:
Inhibitor of DNA binding 4, HLH protein
Alias Symbols:
bHLHb27, BHLHB27, Class B basic helix-loop-helix protein 27, DNA-binding protein inhibitor ID-4, IDB4, Inhibitor of DNA binding 4
NCBI Gene Id:
3400
Protein Name:
DNA-binding protein inhibitor ID-4
Description of Target:
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.
Swissprot Id:
P47928
Protein Accession #:
NP_001537.1
Nucleotide Accession #:
NM_001546.3
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
3.9 pg/mL
Kit Range:
15.6 pg/mL-1,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level ID4 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level ID4 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology ID4 ELISA Kit (Human) (OKCA00660) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ID4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ID4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ID4 captured in the well.
Kit Component:
ComponentAmount
ID4 Microplate96 Wells (12 x 8 Well strips)
ID4 Lyophilized Standard2
100X Biotinylated ID4 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean n=4 (%)Range (%)
Neat9387-97
1:28681-92
1:49690-102
1:8107102-113
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)9284-96
EDTA Plasma (n=4)10496-107
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ID4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ID4.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA

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