ID1 Antibody - middle region (OAAB07962)

Catalog No: OAAB07962

Size:400ul

ID1 Antibody (Center) (Cat. #OAAB07962) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ID1 antibody detected the ID1 protein (arrow).

ID1 Antibody (Center)(Cat. #OAAB07962) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:100) antibody detected the ID1 protein (arrow).

ID1 Antibody (Center) (Cat. #OAAB07962)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ID1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

ID1 Antibody (Center) (Cat. #OAAB07962) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ID1 Antibody (Center)(Cat#OAAB07962) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P, IF, FC
  Function: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).
  Cellular Location: Cytoplasm. Nucleus.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids of human ID1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: ID1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  FC: 1:25

Target Info

• Gene Symbol: ID1
• Alias Symbols: ID, bHLHb24
• NCBI Gene Id: 3397
• Protein Name: DNA-binding protein inhibitor ID-1
• Description of Target: The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
• Uniprot ID: P41134
• Protein Accession #: NP_002156.2
• Molecular Weight: 16133 Da