ICAM2 ELISA Kit (Mouse) (OKCD02607)

Catalog No: OKCD02607

Size:96WELLS

Mouse ICAM2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-97%	80-93%	98-105%	88-95%
  EDTA Plasma (n=5)	87-104%	81-94%	98-105%	88-95%
  Heparin Plasma (n=5)	87-102%	81-89%	78-97%	80-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 2 (ICAM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 2 (ICAM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 2 (ICAM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Intercellular Adhesion Molecule 2 (ICAM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	88-101	97
  EDTA Plasma (n=5)	79-97	92
  Heparin Plasma(n=5)	81-96	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 2 (ICAM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 2 (ICAM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-ICAM2 Microplate: 96 Wells (12 x 8 Well strips)
  • ICAM2 Lyophilized Standard: 2
  • 100X Biotinylated ICAM2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.071 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 2 (ICAM2). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 2 (ICAM2) and analogues was observed
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Icam2
• Gene Full Name: intercellular adhesion molecule 2
• Alias Symbols: CD102;Icam;Icam-2;intercellular adhesion molecule 2;Ly-60;lymphocyte function-associated AG-1 counter-receptor.
• NCBI Gene Id: 15896
• Protein Name: Intercellular adhesion molecule 2
• Description of Target: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
• Uniprot ID: P35330
• Protein Accession #: NP_034624.1
• Nucleotide Accession #: NM_010494.2
• Protein Size (# AA): 277