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Catalog No: OKAA00025-96W
Size:96 Wells
Price: $450.00
SKU
OKAA00025-96W
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit ReproducibilityThe coefficient of variation of both intra-assay and inter-assay were less than 10%.
ELISA Kit Component
Components
2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody
2 vials Streptavidin-HRP solution
4 bottles Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human TGF-beta1 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human TGF-beta1 antibody
Additional InformationSample Type: cell culture supernates, serum, and plasma
::Reagents: REAGENTS
1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human sICAM-1 (8x12)
2. 2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human sICAM-1 monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Typecell culture, supernates, serum, and plasma
Sensitivity31 pg/ml
Predicted Homology Based on Immunogen SequenceHuman
SpecificityThis assay recognizes both natural and recombinant human sICAM-1. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: VCaM-1, L-Selectin, Siglec-2, Siglec-3, Siglec-5, ICaM-2, ICaM-3,
Recombinant mouse: JaM-a, JaM-b, JaM-C, ICaM-2, ICaM-5,
Assay InfoAssay Method: Quantitative Sandwich ELISA
Gene SymbolICAM1
Gene Full Nameintercellular adhesion molecule 1
Alias SymbolsBB2, CD54, P3.58
NCBI Gene Id3383
Protein NameIntercellular adhesion molecule 1
Description of TargetThis gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Uniprot IDP05362
Protein Accession #NP_000192
Nucleotide Accession #NM_000201
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