ICAM1 ELISA Kit (Human) : 48 Wells (OKAA00025-48W)

Catalog No: OKAA00025-48W

Size:48 Wells

Standard curve of ICAM1 protein using OKAA00025_48W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human sICAM-1 antibody
  2 vials Streptavidin-HRP solution
  4 bottles Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  1 bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human sICAM-1 (8x12)
  2. 2 vials human sICAM-1 Standard lyophilized, 4000 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human sICAM-1 monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 31 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human sICAM-1. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: VCaM-1, L-Selectin, Siglec-2, Siglec-3, Siglec-5, ICaM-2, ICaM-3,
  Recombinant mouse: JaM-a, JaM-b, JaM-C, ICaM-2, ICaM-5,
  
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ICAM1
• Gene Full Name: intercellular adhesion molecule 1
• Alias Symbols: BB2, CD54, P3.58
• NCBI Gene Id: 3383
• Protein Name: Intercellular adhesion molecule 1
• Description of Target: This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
• Uniprot ID: P05362
• Protein Accession #: NP_000192
• Nucleotide Accession #: NM_000201