Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD00662 (Formerly GWB-KBBZB3)
Size:96WELLS
Price: $700.00
SKU
OKCD00662
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for ICAM1 ELISA Kit (Dog) (OKCD00662)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-103%88-102%81-99%89-103%
EDTA plasma(n=5)91-101%90-102%78-96%98-105%
heparin plasma(n=5)86-93%80-102%89-97%85-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 1 (ICAM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Intercellular Adhesion Molecule 1 (ICAM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)89-9893
EDTA plasma(n=5)78-10281
heparin plasma(n=5)79-10391
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-ICAM1 Microplate96 Wells (12 x 8 Well Strips)
ICAM1 Lyophilized Standard2 x 100 ng
100X Biotinylated ICAM1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.59 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolICAM1
Gene Full Nameintercellular adhesion molecule 1
Alias SymbolsICAM-1;intercellular adhesion molecule 1;intercellular adhesion molecule 1 (CD54), human rhinovirus receptor.
NCBI Gene Id403975
Protein NameIntercellular adhesion molecule 1
Description of TargetIntercellular adhesion molecule 1
Uniprot IDF1PB95
Protein Accession #NP_001003291
Nucleotide Accession #NM_001003291.1
Protein Size (# AA)531
Write Your Own Review
You're reviewing:ICAM1 ELISA Kit (Dog) (OKCD00662)
Your Rating
We found other products you might like!