ICAM1 Chemi-Luminescent ELISA Kit (Rat) (OKCD03568)

Catalog No: OKCD03568

Size:96WELLS

Rat ICAM1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-101%	87-101%	82-98%	97-104%
  EDTA Plasma (n=5)	82-91%	98-105%	92-99%	87-101%
  Heparin Plasma (n=5)	79-88%	95-103%	98-105%	80-96%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Intercellular Adhesion Molecule 1 (ICAM1) level in the sample or standard.
• ELISA Kit Range: 54.9-40,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-99	95
  EDTA Plasma (n=5)	98-105	101
  Heparin Plasma (n=5)	88-96	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Icam1 Microplate: 96 Wells (12 x 8 Well strips)
  • Icam1 Lyophilized Standard: 2
  • 100X Icam1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.0549 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Icam1
• Gene Full Name: intercellular adhesion molecule 1
• Alias Symbols: CD54;ICAM;ICAM-1;intercellular adhesion molecule 1.
• NCBI Gene Id: 25464
• Protein Name: Intercellular adhesion molecule 1
• Description of Target: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
• Uniprot ID: Q00238
• Protein Accession #: NP_037099
• Nucleotide Accession #: NM_012967