Size:96 Tests
$349.00
In Stock
SKU
OKBB00158
Request Bulk
Order Quote
Aviva's
Satisfaction
Guarantee
Contact Us:
  • Toll Free: (888)880-0001
  • Phone: (858)552-6979
  • Email: info@avivasysbio.com
Shipping Info:
  • $40: Antibody & Protein in US
  • $50: 1-2 Kits in US
  • Contact us for international orders.

ICAM-1 ELISA Kit (Human) (OKBB00158)

Catalog#: OKBB00158
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-ZZD043
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology ICAM1 ELISA Kit (Human) (OKBB00158) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for ICAM1 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, Q28-E480) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ICAM1 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Human ICAM1 captured in well.
Kit Range 156 pg/ml-10,000 pg/ml
Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (ng/mL)1.412.35.21.332.275
St. Dev.0.0480.1060.2650.0850.1270.205
%CV3.404.605.096.395.594.1
Kit Component
ComponentAmount
Lyophilized recombinant mouse Fractalkine standard10 ng/tube x 2
Anti-mouse Fractalkine Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse Fractalkine antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional Information Range: 156pg/ml-10,000pg/ml
:: Principle: Aviva’s human ICAM-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ICAM-1 has been precoated onto 96-well plates. Standards (NSO,Q28-E480) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ICAM-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the
human ICAM-1 amount of sample captured in plate.
:: Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, consisting of 455 amino acids with the molecular mass of 50kDa.
Reconstitution and Storage Store as indicated in product manual.
Sample Type cell culture supernates, serum and plasma (heparin, EDTA)
Sensitivity < 10 pg/ml
Predicted Homology Based on Immunogen Sequence No detectable cross-reactivity with any other cytokine.
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Target Reference 1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. Assignment of the gene for intercellular adhesion
molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991.
2. Bella, J.; Kolatkar, P. R.; Marlor, C. W.; Greve, J. M.; Rossmann, M. G. The structure of the two amino-terminal
domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc.
Nat. Acad. Sci. 95: 4140-4145, 1998.
3. Simmons, D.; Makgoba, M. W.; Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell
adhesion molecule NCAM. Nature 331: 624-627, 1988.
Specificity There is no detectable cross-reactivity with other relevant proteins.
Gene Symbol ICAM1
Official Gene Full Name intercellular adhesion molecule 1
Alias Symbols Intercellular adhesion molecule 1, Major group rhinovirus receptor, CD54
NCBI Gene Id 3383
Protein Name Intercellular adhesion molecule 1
Description of Target Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1
Swissprot Id P05362
Protein Accession # NP_000192.2
Nucleotide Accession # NM_000201.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express ICAM1.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express ICAM1.
Write Your Own Review
You're reviewing:ICAM-1 ELISA Kit (Human) (OKBB00158)
Your Rating