Catalog No: OKCD07132
Size:96WELLS
Price: $750.00
SKU
OKCD07132
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Datasheets/ManualsPrintable datasheet for IBSP ELISA Kit (Human) (OKCD07132)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Sialoprotein (BSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Sialoprotein (BSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Sialoprotein (BSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Sialoprotein (BSP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range125-8,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Sialoprotein (BSP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Sialoprotein (BSP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
IBSP Microplate96 Wells (12 x 8 Well strips)
IBSP Lyophilized Standard2
100X IBSP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 51pg/mL
Gene SymbolIBSP
Gene Full Nameintegrin binding sialoprotein
Alias SymbolsBNSP;bone sialoprotein 2;bone sialoprotein II;BSP;BSP II;BSP-II;cell-binding sialoprotein;Integrin-binding sialoprotein;SP-II.
NCBI Gene Id3381
Protein NameBone sialoprotein 2
Description of TargetBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Uniprot IDP21815
Protein Accession #NP_004958.2
Nucleotide Accession #NM_004967.3
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