IAPP ELISA Kit (Rat) (OKEH03223)

Catalog No: OKEH03223

Size:96T

Rat IAPP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology IAPP ELISA Kit (Rat) (OKEH03223) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat Iapp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Iapp and incubated. The Iapp found in the sample or standards competes with the biotinylated Iapp for limited binding sites on the immobilized anti-Rat Iapp antibody. Excess unbound biotinylated Iapp and sample or standard Iapp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Iapp captured in the well and inversely proportional to the amount of Iapp which was contained in the sample or standard.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Component:
  • Anti-Iapp Microplate: 96 Wells (12 x 8 Well strips)
  • Iapp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Iapp-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39 pg/mL
• Specificity: Natural and recombinant Rat Islet amyloid polypeptide
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Iapp
• Gene Full Name: islet amyloid polypeptide
• Alias Symbols: amylin;DAP;diabetes-associated peptide;islet amyloid polypeptide.
• NCBI Gene Id: 24476
• Protein Name: P12969
• Description of Target: Islet amyloid polypeptide
• Uniprot ID: https://www.uniprot.org/uniprot/P12969
• Protein Accession #: NP_036718.1
• Nucleotide Accession #: NM_012586.2
• Protein Size (# AA): 93
• Molecular Weight: 10 kDa