HYAL1 Chemi-Luminescent ELISA Kit (Human) (OKCD05563)

Catalog No: OKCD05563

Size:96WELLS

HYAL1 Chemi-Luminescent ELISA Kit (Human) (OKCD05563) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase (HAase). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hyaluronidase (HAase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hyaluronidase (HAase) level in the sample or standard.
• ELISA Kit Range: 0.69-500ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronidase (HAase) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronidase (HAase) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HYAL1 Microplate: 96 Wells (12 x 8 Well strips)
  • HYAL1 Lyophilized Standard: 2
  • 100X HYAL1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 9 mL
  • Substrate Diluent: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.28ng/mL

Target Info

• Gene Symbol: HYAL1
• Gene Full Name: hyaluronidase 1
• Alias Symbols: HYAL-1;hyaluronidase-1;hyaluronoglucosaminidase 1;Hyaluronoglucosaminidase-1;luCa-1;LUCA1;lung carcinoma protein 1;MPS9;NAT6;plasma hyaluronidase;tumor suppressor LUCA-1.
• NCBI Gene Id: 3373
• Protein Name: Hyaluronidase-1
• Description of Target: May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
• Uniprot ID: Q12794
• Protein Accession #: NP_149349.2
• Nucleotide Accession #: NM_033159.3