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Catalog No: OPCA03874
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OPCA03874
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HXK1 Recombinant Protein (Saccharomyces cerevisiae) (OPCA03874)

Datasheets/ManualsPrintable datasheet for OPCA03874
Product Info
Predicted Species ReactivitySaccharomyces cerevisiae
Product FormatLiquid
Additional InformationSpecies Specificity Detail: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Reconstitution and StorageBriefly centrifuge lyophilized product prior to opening to bring the contents to the bottom. Please reconstitute protein to 0.1-1.0 mg/mL by adding deionized sterile water first, followed by addition of glycerol to a final concentration of 5-50%. Reconstituted product should be aliquoted for long-term storage at -20C/-80C. Our in house default final concentration of glycerol is 50% for reference. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
FormulationTris-base, 50% glycerol
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequencePartial: VHLGPKKPQARKGSMADVPKELMDEIHQLEDMFTVDSETLRKVVKHFIDELNKGLTKKGGNIPMIPGWVMEFPTGKESGNYLAIDLGGTNLRVVLVKLSGNHTFDTTQSKYKLPHDMRTTKHQEELWSFIADSLKDFMVEQELLNTKDTLPLGFTFSYPASQNKINEGILQRWTKGFDIPNVEGHDVVPLLQNEISKRELPIEIVALINDTVGTLIASYYTDPETKMGVIFGTGVNGAFYDVVSDIEKLEGKLADDIPSNSPMAINCEYGSFDNEHLVLPRTKYDVAVDEQSPRPGQQAFEKMTSGYYLGELLRLVLLELNEKGLMLKDQDLSKLKQPYIMDTSYPARIEDDPFENLEDTDDIFQKDFGVKTTLPERKLIRRLCELIGTRAARLAVCGIAAICQKRGYKTGHIAADGSVYNKYPGFKEAAAKGLRDIYGWTGDASKDPITIVPAEDGSGAGAAVIAALSEKRIAEGKSLGIIGA
Storage BufferTris-base, 50% glycerol
SourceE.coli
TagN-terminal 6xHis-tagged
ReferenceGlobal analysis of Cdk1 substrate phosphorylation sites provides insights into evolution.Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.Science 325:1682-1686(2009)
Gene SymbolHXK1
Alias SymbolsHexokinase PIHexokinase-A
NCBI Gene Id850614
Protein NameHexokinase-1
Uniprot IDP04806
Protein Accession #NP_116711
Nucleotide Accession #NM_001180018
Molecular Weight58 kDa
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