Catalog No: OKEH01643
Size:96T
Price: $525.00
SKU
OKEH01643
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Rat, Rattus norvegicus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Competitive | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology HUWE1 ELISA Kit (Rat) (OKEH01643) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat HUWE1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HUWE1 and incubated. The HUWE1 found in the sample or standards competes with the biotinylated HUWE1 for limited binding sites on the immobilized anti-rat HUWE1 antibody. Excess unbound biotinylated HUWE1 and sample or standard HUWE1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HUWE1 captured in the well and inversely proportional to the amount of HUWE1 which was contained in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.6% (n = 20) Mean Inter-assay CV%: < 9.6% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.406 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Rat E3 ubiquitin-protein ligase HUWE1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Competitive ELISA |
Gene Symbol | HUWE1 |
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Gene Full Name | HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase |
Alias Symbols | HECT, UBA and WWE domain-containing protein 1;HECT-type E3 ubiquitin transferase HUWE1;Upstream regulatory element-binding protein 1. |
NCBI Gene Id | 501546 |
Protein Name | P51593 |
Description of Target | E3 ubiquitin-protein ligase HUWE1 |
Uniprot ID | https://www.uniprot.org/uniprot/P51593 |
Protein Size (# AA) | 322 |
Molecular Weight | 37 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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