Catalog No: OKEH01642
Size:96T
Price: $525.00
SKU
OKEH01642
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HUWE1 ELISA Kit (Human) (OKEH01642) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HUWE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HUWE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HUWE1 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.1% (n = 20)
ELISA Kit Component
ComponentAmount
HUWE1 Microplate96 Wells (12 x 8 Well strips)
HUWE1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated HUWE1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.067 ng/mL
SpecificityNatural and recombinant Human E3 ubiquitin-protein ligase HUWE1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHUWE1
Gene Full NameHECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1
Alias SymbolsARF-binding protein 1;ARF-BP1;BJ-HCC-24 tumor antigen;E3 ubiquitin-protein ligase HUWE1;HECT domain protein LASU1;HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase;HECT, UBA and WWE domain-containing protein 1;HECTH9;HECT-type E3 ubiquitin transferase HUWE1;homologous to E6AP carboxyl terminus homologous protein 9;HSPC272;Ib772;large structure of UREB1;LASU1;Mcl-1 ubiquitin ligase E3;MRXST;MULE;upstream regulatory element-binding protein 1;UREB1;URE-B1;URE-binding protein 1.
NCBI Gene Id10075
Protein NameQ7Z6Z7
Description of TargetE3 ubiquitin-protein ligase HUWE1
Uniprot IDhttps://www.uniprot.org/uniprot/Q7Z6Z7
Protein Accession #NP_113584.3
Nucleotide Accession #NM_031407.6
Protein Size (# AA)4374
Molecular Weight481 kDa
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