Catalog No: OKEH01642
Size:96T
Price: $525.00
SKU
OKEH01642
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology HUWE1 ELISA Kit (Human) (OKEH01642) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HUWE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HUWE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HUWE1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 5.3% (n = 20) Mean Inter-assay CV%: < 9.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.067 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human E3 ubiquitin-protein ligase HUWE1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | HUWE1 |
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Gene Full Name | HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 |
Alias Symbols | ARF-binding protein 1;ARF-BP1;BJ-HCC-24 tumor antigen;E3 ubiquitin-protein ligase HUWE1;HECT domain protein LASU1;HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase;HECT, UBA and WWE domain-containing protein 1;HECTH9;HECT-type E3 ubiquitin transferase HUWE1;homologous to E6AP carboxyl terminus homologous protein 9;HSPC272;Ib772;large structure of UREB1;LASU1;Mcl-1 ubiquitin ligase E3;MRXST;MULE;upstream regulatory element-binding protein 1;UREB1;URE-B1;URE-binding protein 1. |
NCBI Gene Id | 10075 |
Protein Name | Q7Z6Z7 |
Description of Target | E3 ubiquitin-protein ligase HUWE1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q7Z6Z7 |
Protein Accession # | NP_113584.3 |
Nucleotide Accession # | NM_031407.6 |
Protein Size (# AA) | 4374 |
Molecular Weight | 481 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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