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Human TNF ALPHA Protein (OPSA10957)

50 ug
$459.00
In Stock
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Gene Symbol:
TNF
Alias Symbols:
DIF, TNFA, TNFSF2, TNF-alpha, TNF
NCBI Gene Id:
7124
Swissprot Id:
P01375
Protein Accession #:
NP_000585.2
Protein Name:
Tumor necrosis factor
Description of Target:
RECOMBINANT HUMAN TNF ALPHA
Protein Size (# AA):
233
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express TNF.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express TNF.
Product Format:
Purified recombinant cytokine - lyophilised
Predicted Homology Based on Immunogen Sequence:
Human
Predicted Species Reactivity:
Human
Purity:
>98% by SDS PAGE and HPLC analysis
Application Info:
ELISA:    0.2 - 0.4ng/well
Western Blotting:    1.5 - 3.0ng/lane
Functional Assays:    0.05 - 20.0ng/ml
following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
 YesNoNot DeterminedSuggested Dilution
ELISA    0.2 - 0.4ng/well
Western Blotting    1.5 - 3.0ng/lane
Functional Assays    0.05 - 20.0ng/ml
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
Additional Information:
Approx Protein Conc: Total protein concentration 0.1 mg/ml after reconstitution
Buffer solution: 3mM TRIS pH8.0
:::
Endotoxin Level: < 0.1ng/ug
:::
Preservative: None present
Reconstitution and Storage:
Reconstitution: Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently mixed after reconstitution. For
Target Reference:
1. Cernuda-Morollon, E. et al. (2001) 15-Deoxy-Delta 12,14-prostaglandin J2 inhibition of NF-kappaB-DNA binding through covalent modification of the p50 subunit. J. Biol.Chem. 276 (38): 35530 - 35536
2. Taubert, A. & Hermosilla, C. (2008) Bovine recombinant IFNgamma induces endothelial cell gene transcription of immunoregulatory molecules and upregulates PMN and PBMC adhesion on bovine endothelial cells. Vet Res Commun. 32: 35-47.
Datasheets/Manuals:
Printable datasheet for OPSA10957

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