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Human INTERLEUKIN-2 Protein (OPSA10946)

  • Catalog#: OPSA10946
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  • This product is Genway GWB-331A2F
Predicted Species Reactivity:
Human
Product Format:
Purified protein produced in E. coli - lyophilised
Application:
ELISA, WB
Additional Information:
Activity: >1 x 107 units/mg as determined by dose-dependent stimulation of CTLL-2 cells.
Approx Protein Conc: Total protein concentration 0.1mg/ml after reconstitution.
:::
Endotoxin Level: < 0.1 ng/ug
:::
Preservative: None present
Buffer Solution: 10mM Sodium citrate
Reconstitution and Storage:
Reconstitute with 0.1 ml 0.1M acetic acid
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently mixed after reconstitution.
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
Predicted Homology Based on Immunogen Sequence:
Human
Purity:
>98% by SDS PAGE and HPLC sequence analysis
Application Info:
ELISA: 0.2 - 0.4ng/well
This product may be used as a standard for ELISA applications with either OASA07877 or OASA07879
Western Blotting: 1.5 - 3.0ng/lane
This product may be used as a positive control for Western Blotting applications with either OASA07877 or OASA07879
Target Reference:
1. Akari, H. et al. (2000) Nef-induced major histocompatibility complex class I down-regulation is functionally dissociated from its virion incorporation, enhancement of viral infectivity, and CD4 down-regulation. J. Virol. 74 (6): 2907 - 2912.
2. Chan, P.K. et al. (2010) T-cell response to human papillomavirus type 58 L1, E6 and E7 peptides in women with cleared infection, cervical intraepithelial neoplasia and invasive cancer.
Datasheets/Manuals:
Printable datasheet for OPSA10946
Gene Symbol:
IL2
Alias Symbols:
IL-2, TCGF, lymphokine, IL2
NCBI Gene Id:
3558
Protein Name:
Interleukin-2
Description of Target:
RECOMBINANT HUMAN INTERLEUKIN-2
Swissprot Id:
P60568
Protein Accession #:
NP_000577.2
Protein Size (# AA):
15.5kD (134 Amino acid sequence)
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL2.

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