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Human IgG (gamma chain) Antibody - HRP Conjugated (OARA04964)

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Description of Target:
Anti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain). It is a protein complex composed of four peptide chain - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG (gamma chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
Molecular Weight:
40 kDa (label M/w)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Human IgG gamma chain
Product Format:
Lyophilized 0.02M Potassium Phosphate, 0.15M Sodium Chloride (pH 7.2), 10 mg/mL BSA (immunoglobulin & protease free) with 0.01% (w/v) Gentamicin Sulfate
Color: Substrate dependent
Reconstitution and Storage:
Reconstitute with 1.0 mL of deionized water (or equivalent). Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Do NOT add sodium azide!
1.0 mg/ml (by UV absorbance at 280 nm)
Printable datasheet for OARA04964
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Application Info:
ELISA 1:10,000~100,000
WB 1:500~1,000
IHC 1:2,000~10,000
Anti-Human IgG Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

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