Goat Anti-Human IgG (gamma chain) Polyclonal Antibody - HRP Conjugated (OARA04964)

Catalog No: OARA04964 (Formerly GWB-D4DA3E)

Size:1MG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized
• Clonality: Polyclonal
• Isotype: IgG
• Host: Goat
• Conjugation: HRP
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C or -80°C
• Immunogen: Human IgG gamma heavy chain
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Concentration: 1 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
• Application Info: ELISA: 1:10,000~100,000
  WB: 1:500~1,000
  IHC: 1:2,000~10,000
  Optimal dilutions should be determined by the end user.

Target Info

• Description of Target: Anti-Human IgG (gamma chain) Peroxidase generated in goat detects human Immunoglobulin G (gamma chain). It is a protein complex composed of four peptide chain - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG (gamma chain) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.
• Molecular Weight: 40 kDa