Human FADD Antibody : SEPH (OASB01300)

Catalog No: OASB01300 (Formerly GWB-9C49C8)

Size:50T

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 1A11
• Isotype: IgG1
• Host: Mouse
• Conjugation: Sepharose®
• Application: IP
• Additional Information: Description: Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1beta-converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Recombinant FADD
• Concentration: Lot specific
• Specificity: FADD
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by western blotting and/or ELISA toconform with the characteristics of a standard reference reagent.
• Dilution: ELISA: Purified antibody 1:3,000-1:5,000
• Application Info: Western blotting, Immunoprecipitation, Enzyme-Linked-Immunosorbent-Assay (ELISA)
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The Sepharose 4B (SEPH) conjugate is supplied as 50 tests in 2 mL of 100 mM borate buffered saline,pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C. Shake well beforeuse
  - Reagents are stable for the period shown on the label if stored as directed.

Target Info

• Reference: 1. Chinnaiyan, A.M., K. O'Rourke, M. Tewari, and V.M. Dixit. 1995. Cel. 81:505
  2. Cleveland, J.L., and J.N. Ihle. 1995. Cell 81:479
  3. Muzio, M., A.M. Chinnaiyan, F.C. Kishkel, K. O'Rourke, A. Shevchenko, J. Ni, C. Scaffidi, J.D. Bretz, M. Zhang, R. Gentz, M. Mann, P.H
  Krammer, M.E. Peter, and V.M. Dixit. 1996. Cell 85:817
  4. Wieder, T., F. Essmann, A. Prokop, K. Schmelz, K. Schulze- Osthoff, R. Beyaert, B. Dorken, and P.T. Daniel. 2001. Blood 97:1378
  5. Newton, K., A.W. Harris, M.L. Bath, K.G.C. Smith, and A. Strasser. 1998. EMBO J. 17:706
  6. Boldin, M.P., E.E. Varfolomeev, Z. Pancer, I.L. Meet, J.H. Camonis, and D. Wallach. 1995. J. Biol. Chem. 270:7795.
• Gene Symbol: FADD
• Gene Full Name: FAS-associated death domain protein
• Alias Symbols: GIG3, MORT1
• NCBI Gene Id: 8772
• Protein Name: FAS-associated death domain protein
• Description of Target: The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
• Uniprot ID: Q13158
• Protein Accession #: NP_003815.1
• Nucleotide Accession #: NM_003824.3