Human DR5 Antibody (OASB01407)

Catalog No: OASB01407 (Formerly GWB-18A3C7)

Size:100UG

Amount Used: 3 Ug/106 cells Jurkat cells were incubated with the isotype control and purified Rabbit Anti-Human DR5 and then stained with Goat Anti-Rabbit IgG(H+L), human/mouse ads-FITC.

Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Rabbit Anti-Human DR5-UNLB. Proteins were visualized using Goat Anti-Rabbit IgG(H+L), Mouse/Human ads-HRP secondary antibody and chemiluminescent detection.

Human hepatocellular carcinoma cell line Hep G2 was stained with Rabbit IgG-UNLB isotype control and Rabbit Anti-Human DR5-UNLB followed by Donkey Anti-Rabbit IgG(H+L), Mouse/Rat/Human SP ads-BIOT, and DAPI.

Human T cell leukemia cell line Jurkat was stained with Rabbit Anti-Human DR5-UNLB followed by Mouse Anti-Rabbit IgG-PE.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: FC, ICC, WB
• Additional Information: Description: DR5, also known as TRAIL-R2, TRICK2 and KILLER, is a widely expressed type I membrane member of the tumor necrosis (TNF) receptor family. Members of this family contain multiple cysteine-rich repeats in their extracellular domain and while the cytoplasmic region is less conserved, they do contain an intracellular 80 amino acid motif known as the 'death domain' (DD). DR5 is a receptor for the cytotoxic ligand TRAIL which induces apoptosis and it is the cytoplasmic DD that is responsible for transducing the death process. DR5 has also been shown to activate NF-kappaB.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Recombinant DR5
• Purification: Affinity chromatography
• Concentration: 0.5 mg/mL
• Specificity: DR5
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by flow cytometry and/or western blottingto conform with the characteristics of a standard reference reagent.
• Dilution: Western Blotting: Purified antibody 1 - 2 ug/mL
  Flow Cytometry: Purified antibody <= 3 ug/10⁶ cells
  Induction of
  Apoptosis:
  Purified antibody 2.5 ug/mL for 12 hours
  (10-12% in Jurkat cells)
• Application Info: Flow cytometry, Western blotting, Induction of apoptosis
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - Reagents are stable for the period shown on the label if stored as directed.

Target Info

• Gene Symbol: TNFRSF10B
• Gene Full Name: Tumor necrosis factor receptor superfamily member 10B
• Alias Symbols: DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5
• NCBI Gene Id: 8795
• Protein Name: tumor necrosis factor receptor superfamily member 10B
• Description of Target: The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene.
• Uniprot ID: O14763
• Protein Accession #: NP_003833.4
• Nucleotide Accession #: NM_003842.4