Human COLLAGEN Type III Protein (OPRA03253)

Catalog No: OPRA03253

Size:500UG

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid. 0.5 M Acetic Acid
• Host: Human
• Conjugation: Unconjugated
• Application: ELISA|IHC|IP|SDS-PAGE|WB
• Reconstitution and Storage: 2°C to 8°C
• Purification: Human Collagen type III has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
• Concentration: 1 mg/ml
• Specificity: This product reacts with anti-Collagen Type III. Reaction with anti-Collagen I, II, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).
• Application Info: ELISA: 1:10000
  IHC: 1:100~500
  WB: 1:1000
  
• Application Data: Human Collagen type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.Human Collagen type III purified protein standard is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.
• Warning: No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Target Info

• Gene Symbol: COL3A1
• Gene Full Name: collagen type III alpha 1 chain
• Alias Symbols: alpha-1 type III collagen, alpha1 (III) collagen, collagen alpha-1(III) chain, collagen, fetal, collagen, type III, alpha 1, EDS4A, EDSVASC, Ehlers-Danlos syndrome type IV, autosomal dominant, PMGEDSV.
• NCBI Gene Id: 1281
• Protein Name: Collagen alpha-1(III) chain|collagen alpha-1(III) chain preproprotein [Homo sapiens]
• Description of Target: Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
• Uniprot ID: P02461
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000081.1
• Protein Size (# AA): Native