Human Brain Tissue Cell Lysate (OCEG01209)

Catalog No: OCEG01209

Size:100ug

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid 0.063 M Tris-HCl (pH 6.8), 2% SDS, 50mM DTT, 0.002% bromophenol blue with 10% glycerol
• Application: WB
• Reconstitution and Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Purification: Antigen Immunoaffiinity Purification
• Concentration: 2 mg/ml
• Application Info: Human brain tissue lysate is ready to load on SDS-PAGE for Western blotting.
• Preparation: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 g/ml of aprotinin, 5 g/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.