Human AFP Antigen Grade Protein (OPMA04171)

Catalog No: OPMA04171

Size:1MG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid 0.1M Tris, 0.2M Sodium chloride, pH 7.5±0.2, 0.1% Sodium azide
• Application: Western blot
• Reconstitution and Storage: Centrifuge before opening to ensure complete recovery of vial contents. Short-term (up to 14 days) store at 2 to 8°C. Long term, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles.
• Purification: Affinity Purified
• Concentration: Specific Protein: 3.19 mg/ml (Roche Elecsys AFP Assay)
  Total Protein: 3.72 mg/ml (OD280nm, E^0.1%=0.53)
• Purity: > 95% pure (PAGE electrophoresis, Western blot)
• Specificity: AFP has three isoforms (AFP-L1, AFP-L2 and AFP-L3) of which AFP-L3 is the main isoform associated with germ cell and liver tumors. OPMA04171 is derived from a liver tumor cell (not human cord serum) and contains approximately 70% of the AFP-L3 isoform (compared to human cord serum which typically only contains 10% AFP-L3).
• Source: Cell culture derived; Human Hepatocellular Carcinoma.
• Application Info: Suitable for use in Western Blot. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
• Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water.

Target Info

• Gene Symbol: AFP
• Alias Symbols: AFPD, FETA, HPAFP
• NCBI Gene Id: 174
• Protein Name: Alpha-fetoprotein
• Uniprot ID: P02771
• Protein Accession #: NP_001125.1