Catalog No: OPCD04052
Price: $0.00
SKU
OPCD04052
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Datasheets/Manuals | Printable datasheet for HTRA1 Recombinant Protein (OPCD04052) |
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Predicted Species Reactivity | Escherichia coli |
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Product Format | Freeze-dried Powder. PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
Host | E.coli |
Conjugation | Unconjugated |
Application | Positive control|Sodium sodecyl sulfate - polyacrylamide gel electrophoresis|Western blot |
Additional Information | Endotoxin Level: < 1.0 EU per 1 ug (determined by the LAL method) |
:: | Residues: Lys304-Lys442 |
Reconstitution and Storage | 2°C to 8°C|-80°C |
Concentration | 200 ug/mL (prior to lyoph) |
Purity | > 95% |
Source | E.coli |
Tag | N-terminal His Tag |
Gene Symbol | degP |
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Gene Full Name | periplasmic serine endoprotease DegP |
Alias Symbols | b0161;ECK0160;Heat shock protein DegP;htrA;periplasmic serine endoprotease DegP;Protease Do;ptd. |
NCBI Gene Id | 947139 |
Protein Name | Periplasmic serine endoprotease DegP |
Description of Target | DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures (PubMed:10319814). Degrades transiently denatured and unfolded or misfolded proteins which accumulate in the periplasm following heat shock or other stress conditions (PubMed:16303867). DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids (PubMed:8830688). Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins (PubMed:8830688). Its proteolytic activity is essential for the survival of cells at elevated temperatures (PubMed:7557477). It can degrade IciA, Ada, casein, globin and PapA. DegP shares specificity with DegQ (PubMed:8830688). DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP). |
Uniprot ID | P0C0V0 |
Protein Size (# AA) | Lys304~Lys442 |
Molecular Weight | 16kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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