HTR3A Antibody - N-terminal region (AVARP13046_P050)

Catalog#: AVARP13046_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: HTR3A
• Official Gene Full Name: 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic
• NCBI Gene Id: 3359
• Protein Name: 5-hydroxytryptamine receptor 3A
• Swissprot Id: P46098
• Protein Accession #: NP_000860
• Nucleotide Accession #: NM_000869
• Alias Symbols: HTR3, 5HT3R, 5-HT-3, 5-HT3A, 5-HT3R
• Replacement Item: This antibody may replace item sc-30562 from Santa Cruz Biotechnology.
• Description of Target: HTR3A belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Protein Size (# AA): 478
• Molecular Weight: 55kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express HTR3A.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HTR3A
• Predicted Homology Based on Immunogen Sequence: Cow: 78%; Dog: 78%; Horse: 91%; Human: 100%; Mouse: 78%; Rat: 78%
• Peptide Sequence: Synthetic peptide located within the following region: LLLPTLLAQGEARRSRNTTRPALLRLSDYLLTNYRKGVRPVRDWRKPTTV
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: HSPA5; CANX;
• Blocking Peptide: For anti-HTR3A (AVARP13046_P050) antibody is Catalog # AAP30718 (Previous Catalog # AAPP01375)
• Target Reference: Panicker,S., et al., (2004) J. Biol. Chem. 279 (27), 28149-28158
• Publications:

  Rinaldi, A. et al. Serotonin receptor 3A expression in normal and neoplastic B cells. Pathobiology 77, 129-35 (2010). IHC, WB, Cow, Dog, Horse, Human, Mouse, Rat 20516728

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: HTR3A antibody tested with Human Jurkat Cells (AVARP13046_P050)

Product Protocols: HTR3A antibody tested by IHC with human kidney (AVARP13046)