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Catalog No: OKCD01766 (Formerly GWB-KBBOU1)
Size:96WELLS
Price: $725.00
SKU
OKCD01766
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Datasheets/ManualsPrintable datasheet for HTR2C ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 2C (HTR2C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 2C (HTR2C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 2C (HTR2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 5-Hydroxytryptamine Receptor 2C (HTR2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 2C (HTR2C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 2C (HTR2C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Htr2c Microplate96 Wells (12 x 8 Well Strips)
Htr2c Lyophilized Standard2 x 20 ng
100X Biotinylated Htr2c Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine Receptor 2C (HTR2C). No significant cross-reactivity or interference between 5-Hydroxytryptamine Receptor 2C (HTR2C) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHTR2C
Gene Full Name5-hydroxytryptamine receptor 2C
Alias Symbols5-HT2C, 5HT-1C, 5-HTR2C
NCBI Gene Id25187
Protein Name5-hydroxytryptamine receptor 2C
Description of TargetG-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and feeding behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.
Uniprot IDP08909
Protein Accession #NP_036897.2
Nucleotide Accession #NM_012765.3
Protein Size (# AA)460
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