HTR2C Antibody (OAAF02802)

Catalog No: OAAF02802 (Formerly GWB-ASD993)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using 5-HT-2C Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using 5-HT-2C Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human 5-HT-2C.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: 5-HT-2C antibody detects endogenous levels of total 5-HT-2C protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:5000
• Reference: Saltzman A.G., Biochem. Biophys. Res. Commun. 181:1469-1478(1991).
  Stam N.J., Eur. J. Pharmacol. 269:339-348(1994).
  Xie E., Genomics 35:551-561(1996).

Target Info

• Gene Symbol: HTR2C
• Gene Full Name: 5-hydroxytryptamine receptor 2C
• Alias Symbols: 5-HT1C;5-HT2C;5HTR2C;5-HTR2C;5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled;5-hydroxytryptamine receptor 1C;5-hydroxytryptamine receptor 2C;HTR1C;serotonin 5-HT-1C receptor;serotonin 5-HT-2C receptor;Serotonin receptor 2C.
• NCBI Gene Id: 3358
• Protein Name: 5-hydroxytryptamine receptor 2C
• Description of Target: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca(2+) ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.
• Uniprot ID: P28335
• Protein Accession #: NP_000859.1
• Molecular Weight: 51 kDa