HTR2A ELISA Kit (Rat) (OKCA01001)

Catalog No: OKCA01001

Size:96 Wells

Rat HTR2A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	87	82-91
  1:2	104	98-110
  1:4	90	85-94
  1:8	84	80-88

• ELISA Kit Principle: Aviva Systems Biology Htr2a ELISA Kit (Rat) (OKCA01001) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Htr2a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Htr2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Htr2a captured in well.
• ELISA Kit Range: 31.25 pg/mL-2,000 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89	84-96
  EDTA Plasma (n=4)	95	90-103

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Htr2a were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Htr2a were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Htr2a Microplate: 96 Wells (12 x 8 Well strips)
  • Htr2a Lyophilized Standard: 2
  • 100X Biotinylated Htr2a Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 7.8 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HTR2A
• Gene Full Name: 5-hydroxytryptamine receptor 2A
• Alias Symbols: 5Ht-2, 5-HT2A
• NCBI Gene Id: 29595
• Protein Name: 5-hydroxytryptamine receptor 2A
• Description of Target: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
• Uniprot ID: P14842
• Protein Accession #: NP_058950.1
• Nucleotide Accession #: NM_017254.1