Htr2a Antibody - N-terminal region (OAAB19255)

Catalog No: OAAB19255

Size:400ul

Anti-Htr2a Antibody (N-term). ctrlat 1: 2000 dilution + mouse heart lysates
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 53 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P
  Cellular Location: Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, axon Cytoplasmic vesicle. Membrane, caveola. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses.
  Tissue Location Detected in neurons in brain cortex. Detected in adult intestine, especially in mucosal epithelium, longitudinal and circular layers of muscularis externa and myenteric plexuses Highly expressed in Paneth cells, and detected at lower levels in enterocytes (at protein level). Detected in neurons in the brain cortex.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Htr2a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human Htr2a.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: IHC-P: 1:25
  WB: 1:2000
• Reference: Yang W.,et al.J. Neurosci. Res. 33:196-204(1992).
  Fiorica-Howells E.,et al.Am. J. Physiol. 282:G877-G893(2002).
  Becamel C.,et al.J. Biol. Chem. 279:20257-20266(2004).
  Weisstaub N.V.,et al.Science 313:536-540(2006).
  Gonzalez-Maeso J.,et al.Neuron 53:439-452(2007).
  

Target Info

• Gene Symbol: HTR2A
• Alias Symbols: Htr, Htr-, Htr2, Htr-2, 5-HT-2, 5-HT-2A, E030013E04
• NCBI Gene Id: 15558
• Description of Target: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
• Uniprot ID: P35363
• Molecular Weight: 53 kDa