Catalog No: OKCD00702 (Formerly GWB-KBBML8)
Size:96WELLS
Price: $675.00
SKU
OKCD00702
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Datasheets/ManualsPrintable datasheet for HTN1 ELISA Kit (Human) (OKCD00702)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histatin 1 (HTN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histatin 1 (HTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histatin 1 (HTN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histatin 1 (HTN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histatin 1 (HTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histatin 1 (HTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-HTN1 Microplate96 Wells (12 x 8 Well Strips)
HTN1 Lyophilized Standard2 x 100 ng
100X Biotinylated HTN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSaliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.59 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Histatin 1 (HTN1). No significant cross-reactivity or interference between Histatin 1 (HTN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolHTN1
Gene Full Namehistatin 1
Alias SymbolsHIS1;histatin-1;histidine-rich protein 1;post-PB protein;PPB.
NCBI Gene Id3346
Protein NameHistatin-1
Description of TargetHistatins are salivary proteins that are considered to be major precursors of the protective proteinaceous structure on tooth surfaces (enamel pellicle). In addition, histatins exhibit antibacterial and antifungal activities.
Uniprot IDP15515
Protein Accession #NP_002150
Nucleotide Accession #NM_002159.3
Protein Size (# AA)57
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