HSPD1 Recombinant Antibody [3D8] (OACA12837)

Catalog No: OACA12837

Size:100UL

Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue
All lanes: HSPD1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62, 18 kDa
Observed band size: 60 kDa

IHC image of OACA12837 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration

IHC image of OACA12837 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Immunohistochemistry,
  Immunoprecipitation,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from human Hsp60
• Purification: Affinity Purified

Target Info

• Gene Symbol: HSPD1
• Gene Full Name: heat shock protein family D (Hsp60) member 1
• Alias Symbols: 60 kDa chaperonin;60 kDa heat shock protein, mitochondrial;chaperonin 60;CPN60;epididymis secretory sperm binding protein;GROEL;heat shock 60kDa protein 1 (chaperonin);Heat shock protein 60;heat shock protein 65;HLD4;HSP60;HSP-60;HSP65;HuCHA60;mitochondrial matrix protein P1;P60 lymphocyte protein;short heat shock protein 60 Hsp60s1;SPG13.
• NCBI Gene Id: 3329
• Protein Name: 60 kDa heat shock protein, mitochondrial
• Description of Target: Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
• Uniprot ID: P10809