HSPBP1 ELISA Kit (Human) (OKCD00909)

Catalog No: OKCD00909 (Formerly GWB-KBBIQ6)

Size:96WELLS

Human HSPBP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	97-105%	82-97%	87-101%	79-96%
  EDTA plasma(n=5)	80-99%	94-103%	94-103%	82-97%
  heparin plasma(n=5)	80-104%	85-94%	84-104%	97-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Binding Protein 1 (HSPBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Binding Protein 1 (HSPBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Binding Protein 1 (HSPBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock 70kDa Binding Protein 1 (HSPBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-101	92
  EDTA plasma(n=5)	91-102	96
  heparin plasma(n=5)	98-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Binding Protein 1 (HSPBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Binding Protein 1 (HSPBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSPBP1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HSPBP1 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated HSPBP1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.118 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Binding Protein 1 (HSPBP1). No significant cross-reactivity or interference between Heat Shock 70kDa Binding Protein 1 (HSPBP1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HSPBP1
• Gene Full Name: HSPA (Hsp70) binding protein 1
• Alias Symbols: FES1;heat shock protein-binding protein 1;hsp70 interacting protein;hsp70-binding protein 1;Hsp70-binding protein 2;Hsp70-interacting protein 1;Hsp70-interacting protein 2;HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1.
• NCBI Gene Id: 23640
• Protein Name: Hsp70-binding protein 1
• Description of Target: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
• Uniprot ID: Q9NZL4
• Protein Accession #: NP_001123578
• Nucleotide Accession #: NM_001130106.1
• Protein Size (# AA): 362