HSPB8 ELISA Kit (Human) (OKCD00301)

Catalog No: OKCD00301 (Formerly GWB-KBBXG7)

Size:96WELLS

Human HSPB8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 8 (HSPb8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein Beta 8 (HSPb8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 8 (HSPb8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein Beta 8 (HSPb8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein Beta 8 (HSPb8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein Beta 8 (HSPb8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSPB8 Microplate: 96 Wells (12 x 8 Well Strips)
  • HSPB8 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated HSPB8 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein Beta 8 (HSPb8). No significant cross-reactivity or interference between Heat Shock Protein Beta 8 (HSPb8) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HSPB8
• Gene Full Name: heat shock protein family B (small) member 8
• Alias Symbols: alpha-crystallin C chain;CMT2L;DHMN2;E2IG1;E2-induced gene 1 protein;H11;heat shock 22kDa protein 8;heat shock 27kDa protein 8;heat shock protein beta-8;HMN2;HMN2A;HSP22;protein kinase H11;small stress protein-like protein HSP22.
• NCBI Gene Id: 26353
• Protein Name: Heat shock protein beta-8
• Description of Target: Displays temperature-dependent chaperone activity.
• Uniprot ID: Q9UJY1
• Protein Accession #: NP_055180
• Nucleotide Accession #: NM_014365.2
• Protein Size (# AA): 196