Catalog No: OKEH00644
Size:96T
Price: $525.00
SKU
OKEH00644
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology HSPB6 ELISA Kit (Human) (OKEH00644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPB6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPB6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPB6 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
HSPB6 Microplate96 Wells (12 x 8 Well strips)
HSPB6 Lyophilized Standard2
100X Biotinylated HSPB6 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Heat shock protein beta-6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHSPB6
Gene Full Nameheat shock protein family B (small) member 6
Alias Symbolsepididymis luminal protein 55;epididymis secretory sperm binding protein;heat shock 20 kDa-like protein p20;heat shock protein beta-6;heat shock protein family B (small) member B6;heat shock protein, alpha-crystallin-related, B6;HEL55;Hsp20;PPP1R91;protein phosphatase 1, regulatory subunit 91.
NCBI Gene Id126393
Protein NameO14558
Description of TargetHeat shock protein beta-6
Uniprot IDhttps://www.uniprot.org/uniprot/O14558
Protein Accession #NP_653218.1
Nucleotide Accession #NM_144617.2
Protein Size (# AA)160
Molecular Weight17 kDa
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