Catalog No: OKEH07452
Size:96T
Price: $800.00
SKU
OKEH07452
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology HSPB1 ELISA Kit (Pig) (OKEH07452) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPB1 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=4.3% (n = 20)
Mean Inter-assay CV%: <=11.8% (n = 20)
ELISA Kit Component
ComponentAmount
HSPB1 Microplate96 Wells (12 x 8 Well strips)
HSPB1 Lyophilized Standard2
100X Biotinylated HSPB1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
::Subcellular Location: Cytoplasm Nucleus Cytoplasm Cytoskeleton Spindle Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
::Unigene: Ssc.5832
String: 9823.ENSSSCP00000008216
::KEGG: ssc:493184
Pfam: PF00011
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.34ng/mL
SpecificityNatural and recombinant Pig Heat shock protein beta-1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHSPB1
Gene Full Nameheat shock protein family B (small) member 1
Alias Symbols27 kDa heat shock protein 1;heat shock 27 kDa protein;heat shock 27kDa protein 1;heat shock protein beta-1;HSP 27;HSP27.
NCBI Gene Id493184
Protein NameQ5S1U1
Description of TargetHeat shock protein beta-1
Uniprot IDhttps://www.uniprot.org/uniprot/Q5S1U1
Protein Accession #NP_001007519.1
Nucleotide Accession #NM_001007518.1
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