HSPB1 ELISA Kit (Bovine) (OKEH03859)

Catalog No: OKEH03859

Size:96T

Bovine HSPB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSPB1 ELISA Kit (Bovine) (OKEH03859) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPB1 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Component:
  • Anti-HSPB1 Microplate: 96 Wells (12 x 8 Well strips)
  • HSPB1 Lyophilized Standard: 2
  • 100X Biotinylated HSPB1 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.086 ng/mL
• Specificity: Natural and recombinant Bovine Heat shock protein beta-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSPB1
• Gene Full Name: heat shock protein family B (small) member 1
• Alias Symbols: Heat shock 27 kDa protein;heat shock 27 kDa protein 1;heat shock 27kDa protein 1;heat shock protein beta-1;heat shock protein family B member 1 variant 1;heat shock protein family B member 1 variant 2;HSP 27.
• NCBI Gene Id: 516099
• Protein Name: Q3T149
• Description of Target: Heat shock protein beta-1
• Uniprot ID: https://www.uniprot.org/uniprot/Q3T149
• Protein Accession #: NP_001020740.1
• Nucleotide Accession #: NM_001025569.1
• Protein Size (# AA): 201
• Molecular Weight: 22 kDa