HSPA9 Antibody - C-terminal region (ARP30175_P050)

Catalog No: ARP30175_P050

WB Suggested Anti-HSPA9 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysateHSPA9 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

Sample Type: rat brain extract (80ug)
Primary antibody dilution: 2ug/ml
Secondary antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary antibody dilution: 1: 20,000
Image Submitted by: Yuzhi ChenUniversity of Arkansas for Medical Science

Skeletal muscle

Sample Type:
Human brain stem cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
HSPA9: Red DAPI:Blue
Gene Name:
HSPA9
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Sample Type:
Human brain stem cells
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:1000
Color/Signal Descriptions:
HSPA9: Red DAPI:Blue
Gene Name:
HSPA9
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Amount and Sample Type:
500 ug mouse brain homogenate
Amount of IP Antibody:
6 ug
Primary Antibody:
HSPA9
Primary Antibody Dilution:
1:500
Secondary Antibody:
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution:
1:5000
Gene Name:
HSPA9
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, IP, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human HSPA9
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 85%; Dog: 92%; Guinea Pig: 92%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: GENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-HSPA9 (ARP30175_P050) antibody is Catalog # AAP30175 (Previous Catalog # AAPS08906)
• Sample Type Confirmation:

  HSPA9 is strongly supported by BioGPS gene expression data to be expressed in HEK293T

• Other Applications Image 1 Data: IP Suggested Anti-HSPA9 Antibody
  Positive Control: NT2 CELL/BRAIN TISSUE
  
• Reference: Shi,M., (2008) J. Neuropathol. Exp. Neurol. 67 (2), 117-124
• Other Applications Image 2 Data: IP Suggested Anti-HSPA9 antibody
  Titration: 2 ug/ml
  Positive Control: Mouse brain homogenate
  
• Other Applications Image 3 Data: IHC Suggested Anti-HSPA9 antibody
  Titration: 2 ug/ml
  Positive Control: Human brain stem cells
  

Target Info

• Gene Symbol: HSPA9
• Gene Full Name: Heat shock 70kDa protein 9 (mortalin)
• Alias Symbols: CSA, MOT, MOT2, SAAN, CRP40, EVPLS, GRP75, PBP74, GRP-75, HSPA9B, SIDBA4, MTHSP75, HEL-S-124m
• NCBI Gene Id: 3313
• Protein Name: Stress-70 protein, mitochondrial
• Description of Target: HSPA9 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA9 is a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone.CSHL1 is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein plays a role in the control of cell proliferation. It may also act as a chaperone. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants have been observed, but most of them are not thought to be protein-coding.
• Uniprot ID: P38646
• Protein Accession #: NP_004125
• Nucleotide Accession #: NM_004134
• Protein Size (# AA): 679
• Molecular Weight: 75kDa
• Protein Interactions: GPX7; FUS; CDKN2A; UBC; TP53; YKT6; CDKN1A; CDC20; CEP250; TUBGCP2; CEP57; AURKB; TUBG1; AURKA; SUMO2; SUMO3; NEDD1; SPRTN; CEP76; HAUS2; TUBGCP4; STAU1; GRSF1; NEDD8; MDM2; EED; RAP1GDS1; PFAS; GINS3; ATG3; OSGEP; BZW2; ABHD14A; TNFAIP8; FERMT2; AIP; CNB