HSPA8 ELISA Kit (Human) (OKEH04446)

Catalog No: OKEH04446

Size:96T

Human HSPA8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSPA8 ELISA Kit (Human) (OKEH04446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for HSPA8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA8 captured in the well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.1% (n = 20)
  Mean Inter-assay CV%: <= 9.9% (n = 20)
• ELISA Kit Component:
  • Anti-HSPA8 Microplate: 96 Wells (12 x 8 Well Strips)
  • HSPA8 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • HSPA8 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.15 ng/mL
• Specificity: Natural and recombinant Human Heat shock cognate 71 kDa protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSPA8
• Gene Full Name: heat shock protein family A (Hsp70) member 8
• Alias Symbols: constitutive heat shock protein 70;epididymis luminal protein 33;epididymis secretory sperm binding protein Li 72p;Heat shock 70 kDa protein 8;heat shock 70kd protein 10;heat shock 70kDa protein 8;heat shock cognate 71 kDa protein;heat shock cognate protein 54;HEL-33;HEL-S-72p;HSC54;HSC70;HSC71;HSP71;HSP73;HSPA10;LAP1;LAP-1;lipopolysaccharide-associated protein 1;LPS-associated protein 1;NIP71;N-myristoyltransferase inhibitor protein 71.
• NCBI Gene Id: 3312
• Protein Name: P11142
• Description of Target: Heat shock cognate 71 kDa protein
• Uniprot ID: https://www.uniprot.org/uniprot/P11142
• Protein Accession #: NP_006588.1
• Nucleotide Accession #: NM_006597.5
• Protein Size (# AA): 646
• Molecular Weight: 71 KDa