HSPA8 Antibody (OAED00158)

Catalog No: OAED00158

Size:50 ug

Western blot analysis of HSC70/HSP70, pAb:
Lane 1: MW Marker;
Lane 2: 3T3 Cell Lysate;
Lane 3: 3T3 Cell Lysate (heat shocked);
Lane 4: PC-12 Cell Lysate;
Lane 5: PC-12 Cell Lysate (heat shocked);
Lane 6: HeLa Cell Lysate;
Lane 7: HeLa Cell Lysate (heat shocked);
Lane 8: HSP70/HSP72 (human), (recombinant);
Lane 9: HSC70/HSP73 (bovine), (recombinant)

Immunohistochemistry analysis of human spleen, neutrophils tissue stained with HSC70/HSP70, pAb at 10 ug/ml.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Dog|Drosophila|Fish|Guinea Pig|Hamster|Human|Monkey|Mouse|Plant|Porcine|Rabbit|Rat|Sheep|Yeast
• Clonality: Polyclonal
• Host: Rabbit
• Application: Western blot
• Reconstitution and Storage: Long Term Storage : -20C
  Avoid freeze/thaw cycles.
• Immunogen: Synthetic peptide corresponding to a portion of rat Hsp70.
• Purification: Protein A affinity purified.
  
• Specificity: Recognizes human, mouse, rat, bovine, dog, chicken, Drosophila, fish, guinea pig, hamster, monkey, pig, plant, rabbit, sheep and yeast HSC70 (HSP70).
• Formulation: Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
• Application Info: Immunohistochemistry (paraffin sections)
  Western Blot (1:1000, colorimetric)
  Optimal conditions must be determined individually for each application.

Target Info

• Gene Symbol: Hspa8
• Gene Full Name: heat shock protein family A (Hsp70) member 8
• Alias Symbols: Heat shock 70 kDa protein 8;heat shock 70kD protein 8;heat shock 70kDa protein 8;heat shock cognate 71 kDa protein;Heat shock cognate protein 70;heat shock protein 8;heat shock protein A8;Hsc70.
• NCBI Gene Id: 24468
• Protein Name: Heat shock cognate 71 kDa protein
• Description of Target: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle of HSP70, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity of HSP70 for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. HSP70 goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The HSP70-associated co-chaperones are of three types: J-domain co-chaperones HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase STUB1.
• Uniprot ID: P63018
• Protein Accession #: NP_006588.1; NP_694881.1
• Nucleotide Accession #: NM_006597.5; NM_153201.3
• Molecular Weight: Detects a band of ~70kDa by Western blot.