HSPA5 ELISA Kit (Pig) (OKEH03933)

Catalog No: OKEH03933

Size:96T

Pig HSPA5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSPA5 ELISA Kit (Pig) (OKEH03933) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA5 captured in well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Component:
  • HSPA5 Microplate: 96 Wells (12 x 8 Well strips)
  • HSPA5 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated HSPA5 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.064 ng/mL
• Specificity: Natural and recombinant Pig 78 kDa glucose-regulated protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSPA5
• Gene Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• Alias Symbols: 78 kDa glucose-regulated protein;Binding-immunoglobulin protein;Heat shock protein 70 family protein 5;Heat shock protein family A member 5;Immunoglobulin heavy chain-binding protein.
• NCBI Gene Id: 407060
• Protein Name: P34935
• Description of Target: Endoplasmic reticulum chaperone BiP
• Uniprot ID: https://www.uniprot.org/uniprot/P34935
• Protein Accession #: XP_001927830.4
• Nucleotide Accession #: XM_001927795.6
• Protein Size (# AA): 262
• Molecular Weight: 262 kDa