HSPA5 ELISA Kit (Mouse) (OKCD02599)

Catalog No: OKCD02599

Size:96WELLS

Mouse HSPA5 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	87-101%	95-104%	97-104%	81-102%
  EDTA Plasma (n=5)	84-102%	79-97%	83-102%	91-99%
  Heparin Plasma (n=5)	87-101%	92-103%	79-95%	97-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 5 (HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 5 (HSPA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock 70kDa Protein 5 (HSPA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-104	101
  EDTA Plasma (n=5)	86-101	95
  Heparin Plasma(n=5)	80-97	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSPA5 Microplate: 96 Wells (12 x 8 Well strips)
  • HSPA5 Lyophilized Standard: 2
  • 100X Biotinylated HSPA5 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 5 (HSPA5). No significant cross-reactivity or interference between Heat Shock 70kDa Protein 5 (HSPA5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hspa5
• Gene Full Name: heat shock protein 5
• Alias Symbols: 78 kDa glucose-regulated protein;78kD;AL022860;AU019543;B;baffl;baffled;binding-immunoglobulin protein;Bip;D2Wsu141;D2Wsu141e;D2Wsu17;D2Wsu17e;endoplasmic reticulum chaperone BiP;glucose regulated protein, 78 kDa;Grp;Grp78;GRP-78;heat shock 70 kDa protein 5;heat shock 70kD protein 5 (glucose-regulated protein, 78kD);heat shock protein 70 family protein 5;heat shock protein family A member 5;Hsce7;Hsce70;HSP70 family protein 5;immunoglobulin heavy chain-binding protein;mB;mBiP;Sez;Sez7;SEZ-7;XAP-1 antigen.
• NCBI Gene Id: 14828
• Protein Name: Endoplasmic reticulum chaperone BiP
• Description of Target: Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity).
• Uniprot ID: P20029
• Protein Accession #: NP_001156906.1
• Nucleotide Accession #: NM_001163434.1
• Protein Size (# AA): 655