HSPA2 ELISA Kit (Human) (OKEH02038)

Catalog No: OKEH02038

Size:96W

Human HSPA2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology HSPA2 ELISA Kit (Human) (OKEH02038) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA2 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.3% (n = 20)
  Mean Inter-assay CV%: < 7.6% (n = 20)
• ELISA Kit Component:
  • HSPA2 Microplate: 96 Wells (12 x 8 Well strips)
  • HSPA2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated HSPA2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.12 ng/mL
• Specificity: Natural and recombinant Human Heat shock-related 70 kDa protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: HSPA2
• Gene Full Name: heat shock protein family A (Hsp70) member 2
• Alias Symbols: epididymis secretory sperm binding protein, heat shock 70kD protein 2, heat shock 70kDa protein 2, heat shock-related 70 kDa protein 2, HSP70-2, HSP70-3.
• NCBI Gene Id: 3306
• Protein Name: Heat shock-related 70 kDa protein 2
• Description of Target: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365). Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells (By similarity).
• Uniprot ID: P54652
• Protein Accession #: NP_068814.2
• Nucleotide Accession #: NM_021979.3
• Protein Size (# AA): 639
• Molecular Weight: 70 kDa