Catalog No: OKCD07104
Size:96WELLS
Price: $800.00
SKU
OKCD07104
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Datasheets/ManualsPrintable datasheet for HSPA1L ELISA Kit (Human) (OKCD07104)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1 Like Protein (HSPA1L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 1 Like Protein (HSPA1L) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
HSPA1L Microplate96 Wells (12 x 8 Well strips)
HSPA1L Lyophilized Standard2
100X HSPA1L HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.65ng/mL
Gene SymbolHSPA1L
Gene Full Nameheat shock protein family A (Hsp70) member 1 like
Alias Symbolsepididymis secretory sperm binding protein;heat shock 10kDa protein 1-like;heat shock 70 kDa protein 1-Hom;heat shock 70 kDa protein 1L;heat shock 70 kDa protein 1-like;heat shock 70kD protein-like 1;heat shock 70kDa protein 1-like;HSP70-1L;HSP70-HOM;HSP70T;hum70t.
NCBI Gene Id3305
Protein NameHeat shock 70 kDa protein 1-like
Description of TargetMolecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365). Positive regulator of PRKN translocation to damaged mitochondria (PubMed:24270810).
Uniprot IDP34931
Protein Accession #NP_005518.3
Nucleotide Accession #NM_005527.3
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