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HSPA1A ELISA Kit (Bovine) (OKEH00647)

96 Wells
$599.00
In Stock
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Application:
ELISA
Gene Symbol:
HSPA1A
NCBI Gene Id:
281825
Alias Symbols:
Heat shock 70 kDa protein 1, Heat shock 70 kDa protein 1A, HSP70.1, HSP70-1, HSPA1
Protein Name:
Heat shock 70 kDa protein 1A
Swissprot Id:
Q27975
Protein Accession #:
NP_776975.1
Nucleotide Accession #:
NM_174550.1
Predicted Species Reactivity:
Bovine
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.69 ng/mL
Kit Range:
0.78 - 50 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 5.5% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology HSPA1A ELISA Kit (Bovine) (OKEH00647) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA1A captured in well.
Kit Component:
ComponentAmount
HSPA1A Microplate96 Wells (12 x 8 Well strips)
HSPA1A Lyophilized Standard2
100X Biotinylated HSPA1A Detector Antibody1 x 120 µL
100X Avidin-HRP Conjugate1 x 120 µL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express HSPA1A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express HSPA1A.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural bovine Heat shock 70 kDa protein 1A. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
70 kDa
Protein Size (# AA):
641

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