HSP90B1 ELISA Kit (Rat) (OKCD02549)

Catalog No: OKCD02549

Size:96WELLS

Rat HSP90B1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	84-96%	82-97%	79-89%	95-103%
  EDTA Plasma (n=5)	93-101%	88-95%	94-102%	78-105%
  Heparin Plasma (n=5)	92-99%	83-103%	95-105%	89-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1 (HSP90b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	92-101	96
  EDTA Plasma (n=5)	97-104	101
  Heparin Plasma(n=5)	92-105	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSP90B1 Microplate: 96 Wells (12 x 8 Well strips)
  • HSP90B1 Lyophilized Standard: 2
  • 100X Biotinylated HSP90B1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.052 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90kDa Beta 1 (HSP90b1). No significant cross-reactivity or interference between Heat Shock Protein 90kDa Beta 1 (HSP90b1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Hsp90b1
• Gene Full Name: heat shock protein 90 beta family member 1
• Alias Symbols: 94 kDa glucose-regulated protein;endoplasmin;Grp94;GRP-94;heat shock protein 90 kDa beta member 1;heat shock protein 90, beta, member 1;heat shock protein 90kDa beta (Grp94), member 1;Tra1;tumor rejection antigen gp96.
• NCBI Gene Id: 362862
• Protein Name: Q66HD0
• Description of Target: Endoplasmin
• Uniprot ID: https://www.uniprot.org/uniprot/Q66HD0
• Protein Accession #: NP_001012197.2
• Nucleotide Accession #: NM_001012197.2
• Protein Size (# AA): 804