HSP90B1 ELISA Kit (Human) (OKCD06867)

Catalog No: OKCD06867

Size:96WELLS

HSP90B1 ELISA Kit (Human) (OKCD06867) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1 (HSP90b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Beta 1 (HSP90b1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • HSP90B1 Microplate: 96 Wells (12 x 8 Well strips)
  • HSP90B1 Lyophilized Standard: 2
  • 100X HSP90B1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.127ng/mL

Target Info

• Gene Symbol: HSP90B1
• Gene Full Name: heat shock protein 90 beta family member 1
• Alias Symbols: 94 kDa glucose-regulated protein;ECGP;endoplasmin;endothelial cell (HBMEC) glycoprotein;epididymis luminal protein 35;epididymis secretory sperm binding protein Li 125m;GP96;gp96 homolog;GRP94;heat shock protein 90 kDa beta member 1;heat shock protein 90kDa beta (Grp94), member 1;heat shock protein 90kDa beta family member 1;HEL35;HEL-S-125m;stress-inducible tumor rejection antigen gp96;TRA1;tumor rejection antigen (gp96) 1;tumor rejection antigen 1.
• NCBI Gene Id: 7184
• Protein Name: Endoplasmin
• Description of Target: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
• Uniprot ID: P14625
• Protein Accession #: NP_003290.1
• Nucleotide Accession #: NM_003299.2