HSP90B1 Antibody - N-terminal region (OAAB02314)

Catalog No: OAAB02314

Size:400ul

HSP90B1 Antibody (N-term) (Cat. #OAAB02314) western blot analysis in HepG2, CHO, K562, Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).

HSP90B1 Antibody (N-term) (Cat. #OAAB02314) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HSP90B1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of HSP90B1 Antibody (N-term)(Cat#OAAB02314) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

HSP90B1 Antibody (N-term) (Cat. #OAAB02314) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Tested Species Reactivity: Human, Mouse, Hamster
• Predicted Species Reactivity: Rat, Cow, Chicken, Monkey, Pig
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC, IF
• Additional Information: Cellular Location: Endoplasmic reticulum lumen. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This HSP90B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-43 amino acids from the N-terminal region of human HSP90B1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.5 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50
  IF: 1:10~50
  Optimal dilutions should be determined by the end user.
• Other Applications Image 1 Data: HSP90B1 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Target Info

• Gene Symbol: HSP90B1
• Alias Symbols: ECGP, GP96, TRA1, GRP94, HEL35, HEL-S-125m
• NCBI Gene Id: 7184
• Protein Name: Endoplasmin
• Description of Target: HSP90B1 is highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol.
• Uniprot ID: P14625
• Protein Accession #: NP_003290.1
• Molecular Weight: 92 kDa