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Datasheets/Manuals | Printable datasheet for HSP90B Polyclonal Antibody (OABB00299) |
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Tested Species Reactivity | Human, Mouse, Rat |
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Predicted Species Reactivity | Bovine|Human|Rat |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Application | Immunocytochemistry|Immunohistochemistry|Immunohistochemistry-Frozen|Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: Heat shock protein 90kDa beta member 1 (HSP90B1), known also as endoplasmin, GRP94, is a chaperone protein that in humans is encoded by the HSP90B1 gene. It is mapped to chromosome 12q23.3. This gene encodes a member of a family of adenosine triphosphate (ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. |
:: | Background: Heat shock protein HSP 90-beta is a protein that in humans is encoded by the HSP90AB1 gene.[1][2][3]Hsp90β is mapped to chromosome 12q23.3.4The function of Hsp90βincludes assisting in protein folding, cell signaling, and tumor repression. This protein was first isolated by extracting proteins from stressed cells. These cells were stressed by heating, dehydrating or by other means, all of which caused the cell’s proteins to begin to denature.5 |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Rat: Human, Mouse Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat |
Reference | 1. Rebbe NF, Hickman WS, Ley TJ, Stafford DW, Hickman S (Oct 1989). "Nucleotide sequence and regulation of a human 90-kDa heat shock protein gene". J Biol Chem 264 (25): 15006–11. 2. Chen B, Piel WH, Gui L, Bruford E, Monteiro A (Dec 2005). "The HSP90 family of genes in the human genome: insights into their divergence and evolution". Genomics 86 (6): 627–37. 3. "Entrez Gene: HSP90AB1 Heat shock protein 90kDa alpha (cytosolic), class B member 4. Chen, B., Piel, W. H., Gui, L., Bruford, E., Monteiro, A. The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 86: 627-637, 2005. 5. Prodromou C, Panaretou B, Chohan S, Siligardi G, O'Brien R, Ladbury JE, Roe SM, Piper PW, Pearl LH (August 2000). "The ATPase cycle of Hsp90 drives a molecular 'clamp' via transient dimerization of the N-terminal domains". EMBO J. 19 (16): 4383–92.. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Endoplasmin(HSP90B1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat. |
Gene Symbol | HSP90B1 |
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Gene Full Name | heat shock protein 90 beta family member 1 |
Alias Symbols | 94 kDa glucose-regulated protein;ECGP;endoplasmin;endothelial cell (HBMEC) glycoprotein;epididymis luminal protein 35;epididymis secretory sperm binding protein Li 125m;GP96;gp96 homolog;GRP94;heat shock protein 90 kDa beta member 1;heat shock protein 90kDa beta (Grp94), member 1;heat shock protein 90kDa beta family member 1;HEL35;HEL-S-125m;stress-inducible tumor rejection antigen gp96;TRA1;tumor rejection antigen (gp96) 1;tumor rejection antigen 1. |
NCBI Gene Id | 7184 |
Protein Name | Endoplasmin |
Description of Target | Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. |
Uniprot ID | P14625 |
Molecular Weight | 92469 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "HSP90B Polyclonal Antibody (OABB00299)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Bovine|Human|Rat".
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How long will it take to receive "HSP90B Polyclonal Antibody (OABB00299)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "HSP90B Polyclonal Antibody (OABB00299)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "HSP90B Polyclonal Antibody (OABB00299)"?
This target may also be called "94 kDa glucose-regulated protein;ECGP;endoplasmin;endothelial cell (HBMEC) glycoprotein;epididymis luminal protein 35;epididymis secretory sperm binding protein Li 125m;GP96;gp96 homolog;GRP94;heat shock protein 90 kDa beta member 1;heat shock protein 90kDa beta (Grp94), member 1;heat shock protein 90kDa beta family member 1;HEL35;HEL-S-125m;stress-inducible tumor rejection antigen gp96;TRA1;tumor rejection antigen (gp96) 1;tumor rejection antigen 1." in publications.
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What is the shipping cost for "HSP90B Polyclonal Antibody (OABB00299)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "HSP90B Polyclonal Antibody (OABB00299)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "HSP90B Polyclonal Antibody (OABB00299)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "92469 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "HSP90B Polyclonal Antibody (OABB00299)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "HSP90B1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "HSP90B1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "HSP90B1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "HSP90B1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "HSP90B1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "HSP90B1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.