HSP90AB1 ELISA Kit (Bovine) (OKCD01767)

Catalog No: OKCD01767 (Formerly GWB-KBBGJ2)

Size:96WELLS

Bovine HSP90AB1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	87-101%	78-105%	88-96%	79-101%
  EDTA Plasma (n=5)	94-103%	84-104%	93-102%	79-101%
  Heparin Plasma (n=5)	83-101%	88-102%	94-105%	79-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Alpha B1 (HSP90aB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Alpha B1 (HSP90aB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Alpha B1 (HSP90aB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-96	93
  EDTA Plasma (n=5)	91-99	95
  Heparin Plasma (n=5)	82-101	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-HSP90AB1 Microplate: 96 Wells (12 x 8 Well Strips)
  • HSP90AB1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated HSP90AB1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Heat Shock Protein 90kDa Alpha B1 (HSP90aB1). No significant cross-reactivity or interference between Heat Shock Protein 90kDa Alpha B1 (HSP90aB1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: HSP90AB1
• Alias Symbols: HSPCB
• NCBI Gene Id: 767874
• Protein Name: Heat shock protein HSP 90-beta
• Description of Target: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
• Uniprot ID: Q76LV1
• Protein Accession #: NP_001073105.1
• Nucleotide Accession #: NM_001079637.1
• Protein Size (# AA): 724