Catalog No: OKEH06999
Size:96T
Price: $525.00
SKU
OKEH06999
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Hsp90aa1 ELISA Kit (Rat) (OKEH06999) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsp90aa1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hsp90aa1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsp90aa1 captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 109%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <7.1%
Inter-Assay CV <9.9%
ELISA Kit Component
ComponentAmount
Hsp90aa1 Microplate96 Wells (12 x 8 Well strips)
Hsp90aa1 Lyophilized Standard2 x 20 ng
100X Hsp90aa1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.079 ng/mL
SpecificityNatural and recombinant Rat Heat shock protein HSP 90-alpha
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolHsp90aa1, LOC103692716
Gene Full Nameheat shock protein 90 alpha family class A member 1, heat shock protein HSP 90-alpha
Alias Symbolsheat shock 86 kDa;heat shock protein 1, alpha;heat shock protein 86;heat shock protein 90, alpha (cytosolic), class A member 1;heat shock protein 90kDa alpha family class A member 1;heat shock protein HSP 90-alpha;HSP 86;Hsp86;Hsp90;Hspca.
NCBI Gene Id103692716, 299331
Protein NameP82995
Description of TargetHeat shock protein HSP 90-alpha
Uniprot IDhttps://www.uniprot.org/uniprot/P82995
Protein Accession #NP_786937
Nucleotide Accession #NM_175761
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