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Catalog No: OASE00014-HRP
Size:200ug
Price: $519.00
SKU
OASE00014-HRP
Availability: Domestic: within 3-5 days delivery | International: 3-5 days
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Hsp90 complex Antibody : HRP (OASE00014-HRP)

Datasheets/ManualsPrintable datasheet for OASE00014-HRP
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Rabbit
Product FormatLiquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
ClonalityMonoclonal
Clone8D3
IsotypeIgM
HostMouse
ConjugationHRP: Horseradish Peroxidase
ApplicationIP
Additional InformationBackground Info: Immunoprecipitates 90kDa proteins corresponding to the molecular mass of Hsp90. Co-immunoprecipitates Hsp90 complexes, including Hsp70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2alpha (eIF-2alpha) kinase (HRI).
::Scientific Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation.
In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (7).
::Certificate of Analysis: Goat anti-mouse IgM was used to bind 25 ul of protein G-Sepharose. IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 ul of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including hsp90, hsp70 and Hop.
Reconstitution and StorageAll conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
ImmunogenAh receptor (Aryl hydrocarbon receptor)
PurificationPEG Purified
Concentration0.6-0.7 mg/ml
Storage BufferPBS, 50% glycerol
Reference1. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H, et al. (2001) Adv Protein Chem 59:157-186.
3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61.
4. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133.
5. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360.
6. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434.
7. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–
8328.
8. Perdew, G. H. (1988) JBC 263 (27): 13802-13805
9. Dalman, F. C. et al.(1989) JBC 264(33): 19815-19821
10. Uma, S. et al. (1997) JBC 272(17): 11648-11656.
Gene SymbolHSP90AB1
Gene Full Nameheat shock protein 90kDa alpha (cytosolic), class B member 1
Alias SymbolsHSP84, HSPC2, HSPCB, D6S182, HSP90B
NCBI Gene Id3326
Uniprot IDP08238
Protein Accession #NP_031381.2
  1. What is the species homology for "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Rabbit".

  2. How long will it take to receive "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "Hsp90 complex Antibody : HRP (OASE00014-HRP)" provided in?

    This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    This target may also be called "HSP84, HSPC2, HSPCB, D6S182, HSP90B" in publications.

  5. What is the shipping cost for "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Hsp90 complex Antibody : HRP (OASE00014-HRP)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "HSP90AB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "HSP90AB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "HSP90AB1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "HSP90AB1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "HSP90AB1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "HSP90AB1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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